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KMID : 0359319890290040567
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Korean Journal of Veterinary Research
1989 Volume.29 No. 4 p.567 ~ p.575
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Use of the enzyme - linked immunosorbent assay for the detection of toxoplasmosis in swine
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Abstract
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This study was conducted to evaluate the possibility of application of a microenzyme-linked immunosorbent assay (micro-ELISA) for the serodiagnosis of specific toxoplasma antibodies in swine sera and this test was performed as a microplate system by coating the polystyrene plates with toxoplasma soluble antigen, incubated serially diluted sera, then added horse radish peroxidase labelled goat anti-swine IgG(r) conjugate followed by o-phenylenediamine as substrate.
The color development by enzyme-substrate reaction was determined by the photometric reading 〔ELISA reader at 490㎚ (OD)〕and visual reading.
The soluble antigen was prepared from the tachyzoites in mouse peritoneal cavity.
A total of 1,200 swine sera from pig slaughter-house and a total of 116 swine sera from pig breeding station (S-C farm) were tested for the detection of antibodies to Toxoplasma gondii. The results obtained were summarized as follows:
1. The optimal reactions of indirect ELISA for the test sera were determined by the dilution of antigen 1:256 and 1:3,200 of horse radish peroxidase conjugate 〔anti-swine IgG (r)〕.
2. The specific togoplasma antibody (IgG) in pigs infected with Tp artificially were detected as the serum titers of 1:64 or 1:128 at one week postinfection.
3. Of a total of 1,200 swine sera from pig slaughter-house 505 samples of sera were detected as positive (42.1%) and of a total of 116 swine sera from S-C pig breeding station 68 samples of Sera as positive (58.6%).
4. The specific antibody (IgG) detection rates against a total of 1,200 test sera from pig slaughter-house were not significant between male (43.1%) and female (40.7%).
5. The indirect ELISA was proved to be a sensitive and specific procedure for the serodiagnosis of swine toxoplasmosis and also evaluated as an effective screening test for the large scale of test samples in laboratory.
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